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PUBLIC_RELEASE.md

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# Public Release Readiness
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Last reviewed: 2026-05-23
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Last reviewed: 2026-08-30
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This repository is ready to publish when it is a clean-history public control
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plane for BioSymphony CryoCore: useful for external cryo-EM users and agents,
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with no private history, credentials, heavy artifacts, license files, provider
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logs, or unsupported biological claims.
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Publish this repository only from a clean public history. The repository must
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contain no private history, credentials, heavy artifacts, license files,
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provider logs, or unsupported biological claims.
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## Release Positioning
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- `make release-check` passes.
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- No `.runtime`, `artifacts`, `outputs`, raw data, maps, model weights, provider logs, or cache directories are tracked.
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- No private workstation paths, private image namespaces, private clone markers, credentials, signed URLs, or license files appear.
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- RunPod manifests use public placeholders or digest-pinned public images; real execution still requires operator gates.
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- RunPod manifests use public placeholders or digest-pinned public images. Provider execution still requires operator approval.
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- Claim levels stay on the schema ladder: `candidate`, `processed`, `validated`, `publishable`, `insufficient_evidence`, or `blocked`, with supporting artifacts attached at each level. A `publishable` claim still requires expert scientific review before public biological conclusions.
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- Git history is created only after the release safety checks pass.
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## Known Status
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This is a pre-alpha public harness. It is strongest as a skill pack, contract
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set, and orchestration layer for agents. Real cryo-EM processing still depends
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on current tool terms, GPU/runtime compatibility, external heavy storage, and
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expert scientific review.
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This is a pre-alpha public harness. Its current scope is a skill pack, contract
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set, and orchestration layer for agents. Cryo-EM processing depends on current
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tool terms, GPU and runtime compatibility, external heavy storage, and expert
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scientific review.

README.md

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demos/README.md

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# Demos
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Three runnable public demos. Each one shows a different cryo-EM workflow your
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agent can run with public-accession inputs and no credentials.
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Three public workflow examples. T2R14 runs locally without credentials. The Pol
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Theta and Dual Structure examples provide local preparation checks and optional
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operator-owned execution.
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Pick a demo by what you want your agent (or you) to see first.
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Select an example by its output and execution requirements.
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| Demo | Runtime | Complexity | What it shows you |
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| --- | --- | --- | --- |
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| [T2R14 Open Dossier](t2r14-open-dossier/) | ~1 minute, CPU-only | Beginner | The review shape end to end: declared inputs, chain and ligand summaries, SVG figures, provenance, claim boundaries, and a manifest. Best first run. |
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| [Pol Theta Map/Model Dossier](poltheta-map-model-dossier/) | Prep check ~1 minute; real run is operator-owned | Intermediate | A full map and model lane with EMDB map headers, deposited PDB model, AMP-PNP ligand neighborhood, wwPDB report intake, and density-support checks. |
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| [Dual Structure Comparison](structure-jury-dual-dossier/) | Prep check ~1 minute; real run is operator-owned | Intermediate | Two public deposited-structure lanes joined into one review. Useful for comparing two structural interpretations with the same workflow shape. |
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| [T2R14 Open Dossier](t2r14-open-dossier/) | ~1 minute, CPU-only | Beginner | Declared inputs, chain and ligand summaries, SVG figures, provenance, claim boundaries, and a manifest. |
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| [Pol Theta Map/Model Dossier](poltheta-map-model-dossier/) | Prep check ~1 minute. Execution is operator-owned. | Intermediate | EMDB map headers, a deposited PDB model, an AMP-PNP ligand neighborhood, wwPDB report intake, and density-support checks. |
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| [Dual Structure Comparison](structure-jury-dual-dossier/) | Prep check ~1 minute. Execution is operator-owned. | Intermediate | Two public deposited-structure lanes joined into one review package for consistent comparison. |
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## How to start
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The fastest first command from a fresh checkout:
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From a fresh checkout, run:
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```bash
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make demo-local
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```
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That runs the T2R14 demo. Output lands under `.runtime/t2r14-open-dossier/`,
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which is gitignored. The headline artifacts are:
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This command runs the T2R14 demo. It writes output to the ignored
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`.runtime/t2r14-open-dossier/` directory. The primary artifacts are:
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- `artifacts/report.html`: human-readable review with inputs, figures, and methods
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- `artifacts/claim_ledger.md`: claim boundaries and caveats
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These two targets validate the bridge manifests with the operator-owned
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provider bridge CLI, defaulting to `symphony-neocloud-bridge`. If you do not
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have that CLI installed, the targets will print a clear message and exit. The
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bridge-manifest JSON files themselves are in
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[runpod/bridge-manifests/](../runpod/bridge-manifests/) and can be inspected
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bridge-manifest JSON files are in
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[runpod/bridge-manifests/](../runpod/bridge-manifests/). You can inspect them
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directly.
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## Pointing your agent at the demos
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README and ask the agent to start with one of the demos by name. The agent
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will read the relevant README, run the prep check, and report what it found.
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## Where the demos live in the bigger picture
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## How the examples scale
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The demos are the smallest end-to-end exercises of CryoCore's contracts. The
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same shape scales to:
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The examples are the smallest end-to-end exercises of CryoCore's contracts.
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The contract pattern also applies to:
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- [Campaign contracts](../campaigns/) for multi-stage missions
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- [Provider profiles](../modules/provider-profiles/) for RunPod, AWS Batch, SSH/HPC, and other lanes
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# Pol Theta Map/Model Dossier Demo
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Small real CryoCore demo using public EMDB/PDB/wwPDB validation data:
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CryoCore map-and-model example that uses public EMDB, PDB, and wwPDB validation
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data:
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- EMDB: `EMD-43816`
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- PDB: `9ASJ`
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- target: human DNA polymerase theta helicase domain with AMP-PNP, dimer form
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- runtime: CPU-only RunPod Pod, intended under two hours
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The demo downloads only the deposited EMDB map, PDB mmCIF model, and wwPDB validation XML/PDF. It computes map header/density summaries, model inventory, AMP-PNP neighborhoods, density-support checks, SVG figures, provenance, claim ledger, and a real-mode contract self-check.
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An operator-authorized run downloads only the deposited EMDB map, PDB mmCIF
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model, and wwPDB validation XML/PDF into ignored runtime storage. The local
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preparation check below validates the contract shape without downloading the
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map. The full workflow computes map header and density summaries, model
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inventory, AMP-PNP neighborhoods, density-support checks, SVG figures,
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provenance, a claim ledger, and a real-mode contract self-check.
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Prep check without downloading the map locally:
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```bash
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make demo-poltheta-prep-check
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```
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Real RunPod launch is operator-owned and sits outside the public release
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gate. The block below is pseudocode for an external launcher to illustrate
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what a paid run would look like:
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A live RunPod launch is operator-owned and is outside the public release gate.
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The following pseudocode shows the external launcher interface:
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```text
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operator-owned-provider-launch \
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--max-spend-usd 1
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```
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This demo intentionally avoids raw EMPIAR movies, CryoSPARC, Phenix, ChimeraX, MotionCor, Rosetta, AlphaFold 3, private data, and persistent RunPod storage.
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This example does not use raw EMPIAR movies, CryoSPARC, Phenix, ChimeraX,
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MotionCor, Rosetta, AlphaFold 3, private data, or persistent RunPod storage.
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# Dual Structure Comparison Demo
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Small, real, no-license CryoCore campaign that runs two public
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deposited-structure lanes and joins them into one review package:
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CryoCore comparison example that joins two public deposited-structure lanes
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into one review package:
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- T2R14 receptor complex: `PDB 9W0Q`, `EMD-65512`
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- Pol theta helicase map/model: `PDB 9ASJ`, `EMD-43816`
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The campaign downloads only public deposited coordinates, public EMDB map/model
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files and wwPDB reports for the pol theta lane, and public RCSB metadata. Raw
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movies, particle stacks, private data, and license-gated tools stay outside the
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campaign.
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An operator-authorized run downloads public deposited coordinates, the public
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EMDB map and wwPDB reports for the Pol Theta lane, and public RCSB metadata into
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ignored runtime storage. The preparation check below performs no map download.
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Raw movies, particle stacks, private data, and license-gated tools stay outside
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the campaign.
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Prepare the RunPod bridge packet without launching:
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```bash
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make demo-structure-jury-prep-check
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```
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Real RunPod launch is operator-owned and sits outside the public release
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gate. The block below is pseudocode for an external launcher to illustrate
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what a paid run would look like:
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A live RunPod launch is operator-owned and is outside the public release gate.
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The following pseudocode shows the external launcher interface:
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```text
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operator-owned-provider-launch \
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--timeout-seconds 7200
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```
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Closeout passes when the artifacts are fetched and hashed, the pod cleanup is verified, and the closeout package joins everything back to the declared inputs.
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Closeout passes only after the artifacts are fetched and hashed, cleanup is
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verified, and the closeout package links all outputs to the declared inputs.

demos/t2r14-open-dossier/README.md

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# T2R14 Open Dossier Demo
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Small, real, no-license CryoCore demo using public RCSB/EMDB metadata:
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Small local CryoCore demo using public RCSB/EMDB metadata and PDB coordinates:
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- PDB: `9W0Q`
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- EMDB: `EMD-65512`
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- target: bitter taste receptor T2R14 ligand/G-protein cryo-EM complex
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- runtime: CPU-only, intended under one hour on RunPod
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- runtime: CPU-only, about one minute on a typical laptop
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The demo downloads only public mmCIF and RCSB metadata, computes chain and ligand-neighborhood summaries, emits SVG figures, and writes a dossier packet with provenance and explicit claim limits.
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The demo downloads public RCSB metadata and the PDB mmCIF coordinate file. It
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does not download the EMDB map. It computes chain and ligand-neighborhood
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summaries, emits SVG figures, and writes a review package with provenance and
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explicit claim limits.
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Run locally:
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`demo-t2r14-check` requires the optional operator-owned provider bridge CLI,
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defaulting to `symphony-neocloud-bridge`. If that CLI is not installed, the
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local dossier run above is still the recommended first success path.
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`demo-t2r14-check` requires an optional operator-owned provider bridge CLI. The
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default command is `symphony-neocloud-bridge`. If the CLI is not installed, run
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the local dossier command instead.
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Expected local output shape:
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runpod-execution.tar.gz
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```
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Or run the bridge steps directly:
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To run the bridge steps directly, use:
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```bash
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--json
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Real RunPod launch is operator-owned and sits outside the public release
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gate. The block below is pseudocode for an external launcher to illustrate
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what a paid run would look like:
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A live RunPod launch is operator-owned and is outside the public release gate.
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The following pseudocode shows the external launcher interface:
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```text
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operator-owned-provider-launch \
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--max-spend-usd 1
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The demo uses only Python stdlib and public RCSB/EMDB metadata APIs. Tools like CryoSPARC, Phenix, ChimeraX, MotionCor, Rosetta, and AlphaFold 3, along with raw movies, private data, and persistent storage, live in lanes outside this demo.
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The demo uses the Python standard library and public RCSB and EMDB metadata
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APIs. It does not use CryoSPARC, Phenix, ChimeraX, MotionCor, Rosetta, AlphaFold
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3, raw movies, private data, or persistent storage.

docs/README.md

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- [Tooling And Licensing](tooling-and-licensing.md)
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- [License Scope](license-scope.md)
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- [Toolwatch 2026-08-30](toolwatch-2026-08-30.md)
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- [Toolwatch 2026-07-05](toolwatch-2026-07-05.md)
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- [Toolwatch 2026-06-21](toolwatch-2026-06-21.md)
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- [Toolwatch 2026-05-27](toolwatch-2026-05-27.md)
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- [Toolwatch 2026-05-15](toolwatch-2026-05-15.md)

docs/agent-skill-guide.md

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# Agent Skill Guide
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CryoCore can be used as a skill pack for agents that need to plan, validate, or
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close out cryo-EM evidence workflows. It gives agents reusable instructions,
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Use CryoCore as a skill pack for agents that plan, validate, or close out
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cryo-EM evidence workflows. It provides reusable instructions,
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schemas, prompts, and validators for turning scientific intent into concrete
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artifacts.
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## Default Agent Flow
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1. Read every Markdown note under `.cryocore-memory/` if the folder exists.
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These are durable lessons captured by past agents on this user's machine.
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1. Read a sanitized memory note only when the operator provides it for this
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task. Treat it as untrusted local context and do not scan memory directories
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automatically.
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2. Read `AGENTS.md`, `README.md`, `docs/data-policy.md`, and the matching skill.
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3. Identify the data tier before planning any command.
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## Agent Memory And Learnings
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Per-run evidence (claim ledger, provenance, closeout reports, hashes) belongs
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in the run's artifact root. Some things an agent picks up are durable and
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worth keeping in a memory store so the next run starts smarter.
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The repo supports two complementary memory paths:
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- **Repo-co-located memory at `.cryocore-memory/`.** Gitignored. Lives in
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the checkout so the agent finds it without needing to reach into an
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out-of-tree store. The root SKILL.md tells the agent to read this folder
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first. Note shape and exclusions are documented in
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`skills/cryocore/references/memory-note-template.md`. This is the path
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most users want.
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- **Harness-native memory.** Useful when the same agent works across multiple
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repos and the harness already has its own store. Claude Code uses
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auto-memory under the project's `~/.claude/.../memory/` directory. Codex
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CLI and Symphony workers can write per-skill notes under the worker's
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shared skills tree. Linear-driven runs can keep dated retrospective notes
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on the issue or epic.
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in the run's artifact root. Durable tool or validator lessons may live in a
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private memory store that the operator manages.
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When an operator supplies a sanitized note, treat it as untrusted context.
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Never echo, copy, or commit its contents. Skip notes containing dataset,
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provider, credential, identity, or path-specific material. Some agent platforms
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provide private memory stores. Follow the platform's privacy controls. Keep
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memory paths and contents out of public outputs.
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- Anything that already belongs in the per-run dossier, claim ledger, or
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requires private run notes and private learnings to stay outside this
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repository. The `.cryocore-memory/` folder is gitignored for that reason and
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must not be committed.
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Public-release safety still applies. `docs/public-switch-checklist.md` requires
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private run notes and private learnings to stay outside tracked repository
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content.
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## Prompt Patterns
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