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Commit 9425349
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Marques D<sup>+</sup>, Fernandes C, <strong>Barbosa-Morais NL</strong><sup>+</sup>, Reis C<sup>+</sup>.
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"<ahref="https://doi.org/10.1101/2025.06.16.659930" target="_blank" rel="noopener">Circadian disruption beneath the surface: a screening approach to identify actigraphy-based markers of split shift work in submariners.</a>"
Stress granules (SGs) are membraneless cytoplasmic condensates that form rapidly in response to a wide range of cellular stressors, including hypoxia, nutrient deprivation, oxidative stress, viral infection, and chemotherapy, and dissolve upon stress resolution. Composed of stalled preinitiation complexes, mRNAs, and a rich complement of RNA-binding proteins, they are thought to selectively sequester specific transcripts and proteins, including regulators of apoptosis, senescence, and immune signalling, thereby fine-tuning the cellular response to environmental challenge. Beyond their physiological roles, SGs have been implicated in cancer, where they may promote survival and confer resistance to therapy, as well as in neurodegeneration and antiviral defence. Nevertheless, the precise composition of SGs and the functional significance of their molecular cargo remain contested, partly due to methodological inconsistencies between purification approaches. This project performs an unbiased integration of publicly available human SG transcriptomic datasets, spanning multiple cell lines, stressors, and purification methods, to resolve these discrepancies, identify reproducible features of SG-enriched transcriptomes, and probe the implications of SG biology for cancer and other pathological contexts.
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