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Merge pull request #54 from GoekeLab/devel
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‎CHANGELOG.md‎

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@@ -11,7 +11,7 @@ This file contains all notable changes to Bambu-Pipe.
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- Shortened the polyT right flank for 3' and Visium chemistries (`flexiplex_config.csv`) from 30 T to 9 T, matching the flexiplex presets; ONT reads rarely call the full 30 T homopolymer, so few reads matched the flank during barcode discovery
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- Visium samples (`visium-v*`) are now demultiplexed directly against the spot whitelist, as recommended by flexiplex; barcode discovery and flexiplex-filter are skipped, replacing the `-u 0` knee detection workaround
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- Updated the supported chemistry names in the README to the official 10x Genomics assay names, with a link to the 10x Genomics long-read compatibility article
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- flexiplex flank (`-f`) and barcode (`-e`) edit distances are now set per chemistry in `flexiplex_config.csv` (`flank_max_edit_distance`, `barcode_max_edit_distance`), using the flexiplex preset defaults (`-f 8`, `-e 2`); this replaces the `flexiplex_f_5prime`, `flexiplex_f_3prime` and `flexiplex_e` developer parameters. The previous `-f 13` for 3' and Visium chemistries allowed too many errors for the shortened 9 T flank, causing spurious flank matches that slowed `PREPROCESS_FASTQ` considerably
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- Updated the flexiplex flank (`-f`) and barcode (`-e`) edit distances to `-f 8` and `-e 2`, matching the shortened 9 T polyT flank and standardising with the flexiplex documentation; these are now set per chemistry in `flexiplex_config.csv` (`flank_max_edit_distance`, `barcode_max_edit_distance`), replacing the `flexiplex_f_5prime`, `flexiplex_f_3prime` and `flexiplex_e` developer parameters
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- Moved the 10x config files out of `assets/10x_config/` into `assets/`, and renamed `adapter_seq_config.csv` to `cutadapt_config.csv` and `flank_seq_config.csv` to `flexiplex_config.csv`
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