A Python script to calculate:
- GC Content (%)
- DNA Melting Temperature (Tm)
For short sequences (<14 bp): Tm = 2(A + T) + 4(G + C)
For sequences ≥14 bp: Tm = 64.9 + 41(G + C - 16.4) / N
Assumes standard salt concentration (~50 mM Na+).
Sequence: ATGCGTAACGTTAGCCGAT
Length: 19 bp
GC Content: 47.37%
Melting Temperature (Tm): 48.93°C