Automated Blot Quantification

Code I wrote for two implementations of the same automated western blot quantification method: Western Blot Quantification (Unity/C#) and JBlotQuant (ImageJ).

Quick start

See DEPENDENCIES.md for the ImageJ and Unity/OpenCV for Unity requirements.

Repository contents

• JBlots.java, j_blots.jar -- JBlotQuant, the ImageJ plugin (source and compiled build).
• WesternBlotSegmentationAnalysis.cs -- Western Blot Quantification, the Unity C# code.
• media/ -- an example screenshot.
• License: see LICENSE -- research/educational use.

About

Western Blot Quantification is the C# code I wrote for the Unity game engine; JBlotQuant is the ImageJ Java plug-in Nik Mihaylov wrote under my guidance based on the algorithmic steps I designed (see a screenshot showing the software functionality in media/).

For detailed information, see: https://www.researchgate.net/publication/382593670_Quantitative_Video_Microscopy_in_Medicine