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You can use ABBA by activating the PTBIOP update site, but more functionalities are acessible through other installation means. Check the full documentation for instructions.
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## [ABBA](https://www.youtube.com/watch?v=8haRfsY4-_s) - Aligning Big Brains & Atlases
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Aligning Big Brains & Atlases or ABBA for short, is a Fiji plugin which allows to register thin serial sections to several atlases, in coronal, sagittal and horizontal orientations.
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Aligning Big Brains & Atlases, or ABBA for short, is a Fiji plugin to register thin serial sections of brains to
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3D atlases, in coronal, sagittal and horizontal orientations.
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Within Fiji, you have access to the [3D mouse Allen Brain atlas](http://atlas.brain-map.org/atlas?atlas=602630314), and the [Waxholm Space Atlas of the Sprague Dawley Rat Brain](https://www.nitrc.org/projects/whs-sd-atlas). With [ABBA-Python](https://github.com/NicoKiaru/ABBA-Python), you can access all [BrainGlobe atlases](https://brainglobe.info/).
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ABBA is typically used in conjunction with [QuPath](https://qupath.github.io): a QuPath project can serve as an input for ABBA, and the registration results can be imported back into QuPath for downstream processing.
***Atlases**: the [Allen mouse brain atlas](http://atlas.brain-map.org/atlas?atlas=602630314) (CCFv3), the
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[Waxholm Space atlas of the Sprague Dawley rat brain](https://www.nitrc.org/projects/whs-sd-atlas), and all the
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[BrainGlobe atlases](https://brainglobe.info/), opened directly in Fiji.
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***Automated registration**: [DeepSlice](https://github.com/PolarBean/DeepSlice) positions the sections along
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the atlas, [elastix](https://elastix.dev/) refines each one with affine and spline registrations. Both are
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installed by ABBA itself the first time they are used: nothing else to install or configure.
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***Manual correction**: position sections by hand, and edit any registration, with
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[BigWarp](/plugins/bigwarp) for spline registrations.
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***Large images**: ABBA uses [BigDataViewer](/plugins/bdv) to display and transform multiresolution images, the
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typical output of whole slide imaging, on the fly.
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***QuPath**: a [QuPath](https://qupath.github.io) project can be the input of ABBA, and the registered atlas
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regions are imported back into QuPath for cell detection and counting per region.
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***AI assistant** (experimental): through [abba-mcp](https://github.com/unige-biochem/abba-mcp), an AI
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assistant (in VS Code, Claude, Codex...) can run a registration in ABBA's window, where you can take over at any
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time.
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ABBA uses [BigDataViewer](https://imagej.net/plugins/bdv/index) and [BigWarp](https://imagej.net/plugins/bigwarp) for the display and on-the-fly computation of spline-transformed multiresolution images (typical output of Whole Slide Imaging).
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## Installation
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It has been developed by the [BioImaging & Optics Platform](https://www.epfl.ch/research/facilities/ptbiop/) at EPFL. This page contains the reference documentation of ABBA. If you require additional help, please check the troubleshooting section at the bottom of this page.
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In Fiji, enable the `UNIGE-Biochem`[update site](/update-sites/following), then restart Fiji. ABBA needs Java 21,
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which an up-to-date Fiji has.
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ABBA can also be installed with an AI assistant, with a standalone installer, or from Python: see the
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