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cff-version: 1.2.0
message: >-
If you use this software, please cite it as below. Please also cite the CellTune
paper (doi:10.1038/s41592-026-03162-2), whose human-in-the-loop workflow this
extension derives functions from. Depending on which features you use, please
also cite the relevant method papers listed under "references".
title: "SP Classify: an active-learning cell classifier extension for QuPath"
abstract: >-
A QuPath extension for classifying cells in highly multiplexed images. It trains two
different gradient-boosted models on manually labelled cells, then uses their disagreement
to pick the most informative cells for review, so a usable classifier can be built from a
few hundred labels rather than thousands.
type: software
authors:
- family-names: Mckay
given-names: Michael
orcid: "https://orcid.org/0009-0002-2447-9506"
affiliation: "Walter and Eliza Hall Institute of Medical Research"
# Concept DOI — always resolves to the latest release. Use this rather than a
# version-specific DOI so citations do not go stale.
doi: 10.5281/zenodo.21782421
version: 0.3.3
date-released: "2026-10-16"
license: GPL-3.0-only
repository-code: "https://github.com/mikemcka/qupath-extension-sp-classify"
keywords:
- QuPath
- multiplexed imaging
- cell classification
- active learning
- spatial biology
- image analysis
- machine learning
- bioimage analysis
# Method papers whose algorithms this extension reimplements. Cite the ones whose
# corresponding feature you actually use in your analysis.
references:
- type: article
title: "CellTune"
notes: "The human-in-the-loop cell classification workflow this extension derives functions from (Keren Lab). Cite whenever you use this extension."
authors:
- name: "Keren Lab"
doi: 10.1038/s41592-026-03162-2
journal: "Nature Methods"
year: 2026
- type: article
title: "Coordinated Cellular Neighborhoods Orchestrate Antitumoral Immunity at the Colorectal Cancer Invasive Front"
notes: "Cite if you use the Cellular Neighborhoods (CN) analysis."
authors:
- family-names: "Schürch"
given-names: "Christian M."
- family-names: "Bhate"
given-names: "Salil S."
- family-names: "Barlow"
given-names: "Graham L."
doi: 10.1016/j.cell.2020.07.005
journal: "Cell"
volume: 182
issue: 5
year: 2020
- type: article
title: "From Louvain to Leiden: guaranteeing well-connected communities"
notes: "Cite if you use graph-based (Leiden) clustering."
authors:
- family-names: "Traag"
given-names: "Vincent A."
- family-names: "Waltman"
given-names: "Ludo"
- family-names: "van Eck"
given-names: "Nees Jan"
doi: 10.1038/s41598-019-41695-z
journal: "Scientific Reports"
volume: 9
year: 2019
- type: article
title: "SCANPY: large-scale single-cell gene expression data analysis"
notes: "Cite if graph-based clustering (which mirrors the scanpy recipe) is central to your analysis."
authors:
- family-names: "Wolf"
given-names: "F. Alexander"
- family-names: "Angerer"
given-names: "Philipp"
- family-names: "Theis"
given-names: "Fabian J."
doi: 10.1186/s13059-017-1382-0
journal: "Genome Biology"
volume: 19
year: 2018
- type: article
title: "Efficient and robust approximate nearest neighbor search using Hierarchical Navigable Small World graphs"
notes: "Cite if you use cohort/all-cells Leiden clustering (which builds an HNSW kNN graph)."
authors:
- family-names: "Malkov"
given-names: "Yu A."
- family-names: "Yashunin"
given-names: "Dmitry A."
doi: 10.1109/TPAMI.2018.2889473
journal: "IEEE Transactions on Pattern Analysis and Machine Intelligence"
volume: 42
issue: 4
year: 2020
- type: article
title: "Single-cell metabolic profiling of human cytotoxic T cells"
notes: "Cite if you use the recommended arcsinh cofactor (0.05) for MIBI mass-spectrometry data."
authors:
- family-names: "Hartmann"
given-names: "Felix J."
- family-names: "Mrdjen"
given-names: "Dunja"
- family-names: "McCaffrey"
given-names: "Erin"
doi: 10.1038/s41587-020-0651-8
journal: "Nature Biotechnology"
volume: 39
year: 2021
- type: article
title: "UniFORM: Towards Universal Immunofluorescence Normalization for Multiplex Tissue Imaging"
notes: "Cite if you use the Batch Normalisation (UniFORM) feature."
authors:
- family-names: "Wang"
given-names: "Kunlun"
- family-names: "Ait-Ahmad"
given-names: "Karim"
- family-names: "Kupp"
given-names: "Sara"
doi: 10.1101/2024.12.06.626879
journal: "bioRxiv"
year: 2024