The single-nucleus RNA-seq analyses validate the purity of FANS-sorted nuclei from human post-mortem cortex and cerebellum across a series of antibody-panel configurations. The pipeline ingests gene-by-barcode count matrices, performs per-sample quality control, clustering and reference-based annotation, and produces the per-sample UMAP, feature and composition figures presented in the paper. Experimental detail — donors, antibodies, gating strategy, library preparation — is described in the Methods section of the paper; this README covers only what is needed to reproduce the computational results.
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