@@ -16,14 +16,21 @@ info:
1616 # Can contain only lowercase letters or underscores.
1717 - name : ratio_inconsistent_peaks
1818 label : Ratio of inconsistent peaks
19- summary : " Ratio of the number of cell‑type marker‑expression peaks between validation and batch‑normalized data."
19+ summary : " Ratio of the number of cell‑type marker‑expression peaks between unintegrated and batch‑normalized data."
2020 description : |
21- The metric compares the number of cell type specific marker expression peaks between the validation and batch- normalized data.
22- The number of peaks is calculated using the `scipy.signal.find_peaks` function.
23- The metric is calculated as the absolute difference between the number of peaks in the validation and batch-normalized data.
21+ The metric compares the number of cell type specific marker expression peaks between unintegrated and batch normalized data.
22+ The number of peaks is calculated using the `scipy.signal.find_peaks` function.
23+ The metric is calculated as the absolute difference between the number of peaks in the unintegrated and batch-normalized data.
2424 The (cell type) marker expression profiles are first smoothed using kernel density estimation (KDE) (`scipy.stats.gaussian_kde`),
2525 and then peaks are then identified using the `scipy.signal.find_peaks` function.
2626 For peak calling, the `prominence` parameter is set to 0.1 and the `height` parameter is set to 0.05*max_density.
27+ Ratio of inconsistent peaks is defined as number of cases where the number of peaks differ between the two splits in the batch
28+ normalized data divided by the total number of cases.
29+ Cases where there are different number of peaks between the two splits in the unintegrated data are ignored from the denominator.
30+ A lower score indicates better performance, means there are less cases with inconsistent peaks after batch correction.
31+ An alternative peak counting method using persistent homology is also implemented for comparison because peak calling
32+ is sensitive to noise and parameter choices.
33+
2734 references :
2835 doi :
2936 - 10.1038/s41592-019-0686-2
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